SCF-HE001-100μg / 询价
SCF-HE001-500μg / 询价
SCF-HE001-500μgx2 / 询价
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Human SCF Protein
Recombinant Human SCF Protein is expressed from E.coli without tag.It contains Glu26-Ala189 [Accession | P21583-1].
The protein has a predicted MW of 18.45 kDa same as Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
Less than 0.02 EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human SCF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human SCF at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test).
Measured in a cell proliferation assay using Mo7e cells. The ED50 for this effect is 1-8 ng/mL (QC Test).
KACTUS GMP grade cytokines are manufactured and tested in accordance with relevant GMP guidelines and regulatory requirements, and are in compliance with ISO 13485:2016 certified quality system.
● Personnel training programs
● Clean rooms and facility
● Facility maintenance and equipment calibration & verification
● Raw material testing and supplier evaluation and approval
● Animal-free manufacturing
● Rigorous quality control
● Stability testing(freeze-thaw and accelerated stability of reconstituted protein; long-term stability in lyophilized state)
● Comprehensive support files, including COA, COO, MSDS, TSE/BSE statement, etc.
We conduct comprehensive quality control testing to ensure that KACTUS GMP grade cytokines meet strict standards for identification, purity, safety, bioactivity and batch-to-batch consistency.
● Identification by Bis-Tris PAGE
● Purity by SEC-HPLC
● Endotoxin level
● Residual host cell protein
● Residual host cell DNA
● Mycoplasma testing
● Sterility testing
● Bioactivity (Specific activity against standards of WHO if applicable)
● Batch-to-batch consistency
Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.
MGF; c-Kit ligand; sKITLG; KITLG; FPH2; KL-1; Kitl; SF; SHEP7; KL
Wehrle-Haller B. The role of Kit-ligand in melanocyte development and epidermal homeostasis. Pigment Cell Res. 2003 Jun;16(3):287-96. doi: 10.1034/j.1600-0749.2003.00055.x. PMID: 12753403.
KACTUS Human SCF Protein is for research or ex vivo cell processing use, not for human or animal in vivo application.
